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JOHN DEERE 542 For Sale, 2020-7-10 · Browse our inventory of new and used JOHN DEERE 542 For Sale near you at TractorHouse.com. Page 1 of 1Axonal debris accumulates in corneal epithelial cells ..., 2020-5-1 · 2.4. Whole mount immunostaining. Eyes were fixed immediately after euthanization in a paraformaldehyde-containing fixative (1x PBS, 1% formaldehyde, 2-mM MgCl2, 5-mM EGTA, 0.02% NP-40) for 1 h and 15 min at 4 °C, followed by two washes for 10 min each in PBS containing 0.02% NP40 at room temperature.